GECO

At the Genome Engineering CoLab (GECO), our aim is to develop genome engineering tools that unravel the complex genetic underpinnings of diseases. We use these tools in collaboration with researchers working to understand and treat a broad range of human diseases, driving medical discovery and the translation of genetic insights into therapeutic solutions.

GECO helps collaborators in experimental design and execution by helping them choose the best model system, perturbation type, challenge, and readout for their goals. Examples of technologies we use are: CRISPR knockout, interference, base editing, CRISPRoff, and ORF overexpression. Typical readouts include amplicon sequencing, FACS, and scRNA-seq (Perturb-seq).

Enhanced Perturb-seq

Direct-capture Perturb-seqRef technology has improved while costs have plummeted. We have had excellent results using enhanced dCas9 repressors and dual-sgRNA vectors.

10X Genomics Xenium

Imaging-based spatial transcriptomics (iST) is a powerful new technology for quantifying RNAs in their native spatial context. We use a 10X Genomics Xenium.

Genome-wide CRISPR KO

We use the Inzolia library, which is a compact, highly efficient enAsCas12a-based system.Ref

multi-guide constructs provide very high-efficiency KO
Includes thousands of paralogs

Genome-wide CRISPRi

GECO provides a functionally validated, genome-wide CRISPRi libraryRef that uses dual-guide constructsRef for enhanced knockdown efficacy. We also use multiple enhanced CRISPR repressorsRef to minimize silencing and toxicity while improving efficacy.

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